高效液相色谱示差折光法检测紫胶桐酸

采用高效液相色谱(high performance liquid chromatography，HPLC)法，利用示差折光检测器(refractive index detector ，RID)测定紫胶桐酸含量。色谱柱为Agilent ZORBAX SB-C18(4.6mm×250mm，5μm)，以V(甲醇):V(水)＝60:40(0.1%三氟乙酸)作为流动相，流速1mL/min，进样体积10μL，柱温30℃。结果表明：紫胶桐酸在0.01～1.0mg/mL呈良好的线性关系，R2＝0.9994；最低检测限为0.008mg/mL；精密度RSD值为0.86%；平均加标回收率为100.23%，RSD值为0.65%。采用HPLC-RID测定紫胶桐酸的含量，操作简单，结果准确，稳定性和可信度好。

Determination of Aleuritic Acid by High Performance Liquid Chromatography with Differential Refractive Index Detector

In this paper, we present a high performance liquid chromatography (HPLC) with differential refractive index detector (RID) method to determine aleuritic acid. Ten mL of the analyte was chromatographically separated on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5μm) using a mobile phase consisting of a mixture of methanol and water (60:40, containing 0.1% trifluoroacetic acid) at a flow rate of 1 mL/min. The column temperature was set as 30 ℃. Aleuritic acid showed a good linear relationship over the concentration range of 0.01-1.0 mg/mL with a correlation coefficient of 0.9994. The limit of detection was 0.008 mg/mL. The precision RSD was 0.86% (n ＝9). The average spike recovery rate was 100.23% with a RSD of 0.65% (n ＝ 9). The method was easy to operate, simple, accurate, stable and reliable.