| HPLC-MS/MS测定玉米中异恶唑草酮及代谢物残留量 |
| |
| 引用本文： | 杨长志,王传松,程阳,刘永,吴岩,魏冬旭. HPLC-MS/MS测定玉米中异恶唑草酮及代谢物残留量[J]. 食品科学, 2011, 32(22): 280-284. DOI: 10.7506/spkx1002-6630-201122060 |
| |
| 作者姓名： | 杨长志 王传松 程阳 刘永 吴岩 魏冬旭 |
| |
| 作者单位： | 黑龙江出入境检验检疫局 |
| |
| 基金项目： | 国家认证认可管理委员会检验检疫行业标准制订项目(2009B412) |
| |
| 摘 要： | 建立玉米中异恶唑草酮及代谢物残留量同时测定的高效液相色谱-串联质谱(high performance liquid chromatorgerphy-mass spectrometry/mass spectrometry，HPLC-MS/MS)分析法。试样用乙酸-乙腈(1:99, V/V)高速匀浆提取、醋酸钠和无水硫酸镁盐析后，提取液经N-丙基乙二胺(PSA)、十八烷基硅烷(ODS)和石墨化炭黑(GCB)净化，除去样品中脂肪和色素等大多数的干扰基质，用高效液相色谱-串联质谱仪检测，外标法定量。异恶唑草酮及代谢物的添加量在0.005～0.100mg/kg范围内，样品平均加标回收率在74.8%～107.2%之间，相对标准偏差为6.45%～11.15%；方法的检测限异恶唑草酮为0.01mg/kg、异恶唑草酮代谢物0.005mg/kg。本方法灵敏、准确、省时和操作简便，适用于玉米中异恶唑草酮及代谢物残留量同时检测和确证。
|
| 关 键 词： | 异恶唑草酮及代谢物 液相色谱-串联质谱 玉米 |
Determination of Isoxaflutole and Its Metabolite Residues in Maize by HPLC-MS/MS |
| |
| YANG Chang-zhi,WANG Chuan-song,CHENG Yang,LIU Yong,WU Yan,WEI Dong-xu. Determination of Isoxaflutole and Its Metabolite Residues in Maize by HPLC-MS/MS[J]. Food Science, 2011, 32(22): 280-284. DOI: 10.7506/spkx1002-6630-201122060 |
| |
| Authors: | YANG Chang-zhi WANG Chuan-song CHENG Yang LIU Yong WU Yan WEI Dong-xu |
| |
| Affiliation: | (Heilongjiang Entry and Exit Inspection and Quarantine Bureau, Harbin 150001, China) |
| |
| Abstract: | An HPLC-MS/MS(high performance liquid chromatography coupled with tandem mass spectrometry) method has been developed for the determination of isoxaflutole and its two metabolite residues in maize.The isoxaflutole and its metabolite residues in samples were extracted into acetic acid-cetonitrile(1:99,V/V) by means of high-speed homogenization,subjected to liquid-liquid partition in the presence of added anhydrous magnesium sulfate and sodium acetate,cleaned up with primary secondary amine(PSA),octadecylsila... |
| |
| Keywords: | isoxaflutole and its metabolites high performance liquid chromatography coupled with tandem mass spectrometry(HPLC-MS/MS) maize |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
