| 不同碳源强化生物除磷系统的PCR-DGGE分析 |
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| 引用本文： | 刘亚男,夏圣骥,于水利.不同碳源强化生物除磷系统的PCR-DGGE分析[J].哈尔滨工业大学学报,2007,39(4):589-593. |
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| 作者姓名： | 刘亚男 夏圣骥 于水利 |
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| 作者单位： | 哈尔滨工业大学,市政环境工程学院,哈尔滨,150090;东华大学环境科学与工程学院,上海,201620;同济大学,污染控制与资源化研究国家重点实验室,上海,200092;哈尔滨工业大学,市政环境工程学院,哈尔滨,150090 |
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| 基金项目： | 东华大学青年教师基金(113-10-0044065) |
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| 摘 要： | 实验考察了不同碳源强化生物聚磷系统(EBPR)中的聚磷菌群,对3个不同碳源(1号实际生活污水、2号葡萄糖和3号乙酸钠)的序批式反应器(SBR)强化生物聚磷系统聚磷菌的16S rDNA特异性聚合酶链式反应(PCR)扩增产物进行了变形梯度凝胶电泳(DGGE)分析.结果表明,以生活污水为碳源的1号反应器具有数量最多的优势种群,而以葡萄糖和乙酸钠为碳源的2号、3号反应器的生物多样性较少.3个反应系统的微生物种群各有异同.具有除磷作用的未被培养细菌(条带3、4,AF527584、AF502204)为3个反应器所共有的生物量较多的优势种群,且其在以生活污水为碳源的1号反应系统中相对生物量最多.
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| 关 键 词： | 强化生物除磷 碳源 PCR-DGGE 聚磷菌 16S rDNA |
| 文章编号： | 0367-6234(2007)04-0589-04 |
| 修稿时间： | 2005-05-31 |
Application of polymerase chain reaction - denaturing gradient gel electrophoresis to analyze biological phosphorus removal systems fed with different carbon sources |
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| LIU Ya-nan,XIA Sheng-ji,YU Shui-li.Application of polymerase chain reaction - denaturing gradient gel electrophoresis to analyze biological phosphorus removal systems fed with different carbon sources[J].Journal of Harbin Institute of Technology,2007,39(4):589-593. |
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| Authors: | LIU Ya-nan XIA Sheng-ji YU Shui-li |
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| Abstract: | The microbial communities of phosphate-accumulating organisms cultivated with different carbon sources were investigated.Three sequencing batch reactors were operated supplied with sewage,glucose and sodium acetate as carbon sources respectively.Based on the results of denaturing gradient gel electrophoresis(DGGE) of polymerase chain reaction-amplified 16S rDNA fragments,it was demonstrated that the system fed with real sewage had the most biodiversity,while the other two fed with pure carbon source had les... |
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| Keywords: | enhanced biological phosphorus removal carbon source PCR-DGGE phosphate-accumulating bacteria 16S rDNA |
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