Evaluation of the antioxidant capacity of lupin sprouts germinated in the presence of selenium |
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Authors: | Danuta Zielinska Juanna Frias Mariusz Konrad Pisku?a Halina Koz?owska Henryk Zielinski Conception Vidal-Valverde |
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Affiliation: | (1) University of Warmia and Mazury in Olsztyn, Plac Lodzki 4, 10-957 Olsztyn, Poland;(2) Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain;(3) Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, P·O. Box 55, 10-747 Olsztyn, Poland |
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Abstract: | Antioxidant capacity of 5 DAS (days after seeding) lupin sprouts germinated in the presence of selenate or selenite was determined
by cyclic voltammetry (CV), Folin-Ciocalteu reagent (FCR) application and photochemiluminescence (PCL) methods. The hydrophilic
(80% methanol) and lipophillic (hexane/methanol) extracts of 5 DAS lupin sprouts germinated in the absence of selenium (control
sprouts) showed higher antioxidant capacity than ungerminated seeds. Five DAS sprouts produced in the presence of higher concentration
of selenate or selenite (6–8 mg/L) showed increased total antioxidant capacity formed by both hydrophilic and lipohillic antioxidants
when evaluated by CV and FCR methods. The hydrophilic extracts from sprouts produced in the presence of low concentration
of selenate (2–6 mg/L) showed a significantly higher antioxidant capacity of water-soluble compounds (PCL ACW) while no changes
were noted in those germinated in the presence of the highest concentration in relation to the control sprouts. In contrast,
the antioxidant capacity of lipid-soluble compounds (PCL ACL) lowered significantly in relation to the control sprouts. The
similar changes in antioxidant capacity of sprouts produced in the presence of selenite were found by both PCL assays. Comparison
of PCL with CV and FCR reducing capacity assays has shown that these methods yielded considerably different chemical information.
Moreover, the changes in total antioxidant and total reducing capacity of sprouts germinated in the presence of either selenate
or selenite provided by CV and FCR assays were higher than those obtained by photochemiluminesce measurements. It can be suggested
to use 6–8 mg/L of selenite rather than selenate in order to obtain a lupin sprouts with high antioxidant capacity. |
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Keywords: | Lupin sprouts Selenate Selenite Antioxidant capacity Cyclic voltammetry Photochemiluminescence |
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