| 高速逆流色谱法分离纯化绿原酸研究 |
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| 引用本文： | 陈德经,韩豪,董博妮. 高速逆流色谱法分离纯化绿原酸研究[J]. 食品科学, 2008, 29(11): 298 |
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| 作者姓名： | 陈德经 韩豪 董博妮 |
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| 作者单位： | 陕西理工学院陕西省资源生物重点实验室，陕西理工学院生物科学与工程学院 |
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| 摘 要： | 利用高速逆流色谱技术分离纯化金银花中的绿原酸。选择正丁醇-冰乙酸-水(4:1:5,V/V)系统来分离,分离结果经高效液相(HPLC)检测纯度达到98.1%,绿原酸的得率为95%。
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| 关 键 词： | 绿原酸 高速逆流色谱 分离 |
Study on Separation and Purification of Chlorogenic Acid with High-speed Countercurrent Chromatography |
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| CHEN De-jing,HAN Hao,DONG Bo-ni. Study on Separation and Purification of Chlorogenic Acid with High-speed Countercurrent Chromatography[J]. Food Science, 2008, 29(11): 298 |
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| Authors: | CHEN De-jing HAN Hao DONG Bo-ni |
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| Affiliation: | CHEN De-jing1,HAN Hao1,DONG Bo-ni2 |
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| Abstract: | High-speed countercurrent chromatography was used to separate chlorogenic acid from 70% ethanol extract of dry Lonicera japonica Thunb.with three different solvent systems.It was found experimentally that the optimal solvent system is composed of butanol,acetic acid and wate,with the ratio of 4:1:5(V/V).The purity of obtained chlorogenic acid is determined as 98.1% by high performance liquid chromatograph(HPLC),and its yield is 95%. |
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| Keywords: | chlorogenic acid high-speed countercurrent chromatography(HSCCC) seperation |
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