Effect of Castration and hormonal supplementation on cholesterol cholelithiasis in the male hamster |
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Authors: | Akira Ohshima Bertram I Cohen Nariman Ayyad Erwin H Mosbach |
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Affiliation: | (1) Departments of Surgery, Beth Israel Medical Center, First Avenue at 16th St., 10003 New York, New York;(2) Albert Einstein College of Medicine, The Bronx, New York;(3) Present address: Department of Surgery 1, Kyushu University School of Medicine, Maidashi, 3-1-1, Higashi-ku, 812 Fukuoka, Japan |
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Abstract: | This study examined the effect of castration and dietary hormonal supplementation on cholesterol cholelithiasis in male hamsters.
Animals fed a standard lithogenic diet developed cholesterol gallstones (17%) after 6 wk, while castrated hamsters did not
form any stones. Addition of a synthetic androgen, methyltestosterone, to the lithogenic diet induced cholelithiasis in castrated
animals (50%). The biles of normal and castrated-hormone supplemented hamsters had cholesterol saturation indices of 1.0 and
1.1, respectively, while the bile of the castrated animals remained unsaturated (0.6). The ratio of cholic acid/chenodeoxycholic
acid in bile increased after castration, but returned to normal levels following hormonal supplementation. Biliary cholesterol
carriers were separated by ultracentrifugation. Animals in the stone-forming groups (normal and castrated-hormone treated)
had a significant proportion of their biliary cholesterol in vesicles (44 and 46%, respectively); castrated hamsters had less
cholesterol in vesicle form (9%). The molar ratio of cholesterol/phospholipid in vesicles was reduced after castration (0.93
vs. 0.42) and increased by hormonal supplementation (1.89). In conclusion, when compared to normal male hamsters fed a standard
lithogenic diet, castration reduced the cholesterol saturation of bile, lowered the vesicular/micellar ratio in bile, and
inhibited cholesterol cholelithiasis. Dietary androgen supplementation increased the lithogenicity of bile, resulting in stone
formation in castrated animals. |
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