家蝇幼虫培养及其抗菌肽的初步研究

通过天然家蝇幼虫的人工培养及Tricine-SDS-PAGE电泳,分离纯化出2种抗菌肽（命名为MDL-1、MDL-2）,并检测了该抗菌肽的抑菌活性.MDL-1的相对分子量10772Da,MDL-2的相对分子量6091Da.实验结果表明：剩肉菜（骨头和虾皮等）对天然家蝇的产卵引诱力最大;红糖和奶粉的最佳比例为3：1;家蝇幼虫食料最佳含水量为80%;家蝇幼虫单体平均增重最大的最佳培养基为麦麸和骨头的混合物.抑菌活性检测结果：MDL-1对枯草芽胞杆菌的抑菌效果最强,大肠杆菌次之,金黄色葡萄球菌最差;MDL-2对大肠杆菌的抗菌效果最好,金黄色葡萄球菌次之,枯草芽胞杆菌稍差.

Initial Study on Cultivation of Musca domestica Larvae and Its Antibacterial Peptides

The research by man-made cultivation of natural Musca domestica showed leftover meat (bone and skin shrimp) seduced the spawning of natural Musca domestrica maximal extent, and the optimum proportion of brownsuger and powdered milk was 3:1. The optimum moisture of feed which adapted to the growing of Musca domestrica larvae was 80%, and the optimum culture in which the average weight increased of single natural Musca domestrica larvae was max was mixture which contained bone and wheat bran. Two antibacterial peptides that named MDL-1 and MDL-2 were isolated from larvae hemolymph by means of electrophoretic preparation. The molecule weight of antibacterial peptide 1 (MDL-1) was 10772Da, and MDL-2 was 6091Da,and antibacterial peptides had stronger inhibition of fungi, such as E.coli, S.aureus and B.cereus. MDL-1 had the highest activity against B.cereus, higher activity against E.coli, activity against S.aureus. MDL-2 had the strongest potential activity against E.coli, had stronger potential activity against S.aureus, against B.cereus weakly.