不同处理对猪毛角蛋白提取效果及功能特性的影响

目的 探究不同处理方式对猪毛角蛋白提取效果及功能特性的影响。方法 采取碱解法、酶解法、蒸汽爆破法、还原法4种处理提取猪毛角蛋白,研究不同处理对猪毛溶解率和可溶性蛋白含量以及角蛋白功能特性的影响。结果 碱解和蒸汽爆破处理下猪毛溶解率较高,均为65%左右,而还原法处理的可溶性蛋白含量较高,为165mg/g左右;不同处理对猪毛角蛋白的功能特性影响差异较大,溶解性和持水性较好的分别是碱解和蒸汽爆破处理得到的角蛋白,而还原法提取出的猪毛角蛋白持油性、冻融稳定性、乳化性及乳化稳定性较好。结论 各处理方法均有利弊,需要根据角蛋白的用途选择合适的提取方法。本研究为猪毛角蛋白的提取及猪毛的充分利用提供了一定理论参考。

Effects of different treatments on extraction effects and functional properties of keratin from pig hair

ABSTRACT: In order to improve the utilization value of pig hair, four methods were adopted to extract keratin from pig hair, including alkaline hydrolysis, biological method, mechanical method and reduction method, the effects of different treatments on the solubility, soluble protein content and keratin functional properties of pig hair were studied. The results showed that the solubility of pig hair was higher under alkaline hydrolysis and steam blasting treatment, while the soluble protein content was higher under reduction treatment. The effects of different treatments on the functional properties of pig hair keratin are quite different. The keratin obtained by alkaline hydrolysis and steam blasting has the best solubility and water retention, respectively, while the pig hair keratin extracted by reduction method has better oil retention, freeze-thaw stability, emulsification and emulsification stability, so it is necessary to select an appropriate extraction method according to the application path of keratin. This study provides a theoretical reference for the extraction of pig hair keratin and the full utilization of pig.