Quantitative analysis of patulin in apple juice by thin-layer chromatography using a charge coupled device detector |
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Authors: | Juliane Elisa Welke Michele Hoeltz Horacio Alberto Dottori Isa Beatriz Noll |
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Affiliation: | 1. Institute of Food Science and Technology, Federal University of Rio Grande do Sul , Av. Bento Gon?alves 9500, 91570-901 Porto Alegre, RS, Brazil juliwelke@yahoo.com.br;3. Institute of Food Science and Technology, Federal University of Rio Grande do Sul , Av. Bento Gon?alves 9500, 91570-901 Porto Alegre, RS, Brazil;4. Institute of Physics, Federal University of Rio Grande do Sul , Av. Bento Gon?alves 9500, 91570-901 Porto Alegre, RS, Brazil |
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Abstract: | A method was developed and validated in-house for the detection and quantification of patulin in apple juice concentrate using a charge coupled device (CCD) on thin-layer chromatography (TLC) plates. Samples were extracted with ethyl acetate and then cleaned-up by extraction with a sodium carbonate solution. The method showed a mean recovery of 95%. The quantification and detection limit were 14 µg l?1 and 0.005 µg per spot, respectively. The CCD camera is sufficiently sensitive to detect changes in spot fluorescence intensity caused by small differences in mycotoxin concentration under homogeneous illumination from a UV light source. The results of validation confirmed the efficiency of the method, which is sensitive enough to be used to quantify patulin in apple juice by producers or for government monitoring/survey programs. The method was applied to the analysis of 16 apple juice concentrate samples and patulin levels ranged from 15 to 46 µg l?1. This study demonstrated the applicability of the TLC–CCD technique as a tool for monitoring patulin in apple juice. |
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Keywords: | apple juice charge-coupled device imaging patulin thin-layer chromatography |
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