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Rapid determination of polybrominated diphenyl ethers (PBDEs) in fish using selective pressurized liquid extraction (SPLE) combined with automated online gel permeation chromatography-gas chromatography mass spectrometry (GPC-GC/MS) |
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| Authors: | YP Liu YF Zhao YN Wu LY Zhu |
| Affiliation: | 1. National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention , Beijing, China;2. College of Environmental Science and Engineering, Nankai University , Tianjin, China;3. National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention , Beijing, China;4. College of Environmental Science and Engineering, Nankai University , Tianjin, China |
| Abstract: | A rapid method for the determination of seven predominant polybrominated diphenyl ethers (PBDEs) in fish was developed. The analysis could be completed in 1 h. The PBDEs in the sample were extracted with fat by selective pressurized liquid extraction (SPLE) using acid alumina as a fat retainer. After concentration, the extract was directly injected into online gel permeation chromatography with gas chromatography-mass spectrometry (GPC-GC/MS) in negative chemical ionization (NCI) mode. Method detection limits of seven PBDEs were between 0.002 and 0.005 ng g?1 fat. Quantitative recoveries ranging from 81.9% to 110.3% were obtained by analysis of spiked fish. The relative standard deviations were less than 15% (n = 6). The method was applied to a certified reference material of fish; all results were in the reference ranges. It was shown to be a rapid and reliable alternative in the routine analysis for PBDEs. |
| Keywords: | polybrominated diphenyl ethers (PBDEs) selective pressurized liquid extraction (SPLE) gel permeation chromatography-gas chromatography mass spectrometry (GPC-GC/MS) negative chemical ionization (NCI) fish |