Impact of durum wheat milling on deoxynivalenol distribution in the outcoming fractions |
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Authors: | G. Ríos N. Zakhia-Rozis M. Chaurand F. Richard-Forget M.F. Samson J. Abecassis |
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Affiliation: | 1. INRA, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, 2 Place Pierre Viala, F-34060 Montpellier Cedex1, France;2. CIRAD, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, TA 40/16 73 Rue JF Breton, 34398 Montpellier Cedex 5, France;3. INRA, U.R. 1264, Mycologie et Sécurité des Aliments, Domaine de la Grande Ferrade, 71 Av. Edouard Bourleaux, BP 81, 33883 Villenave-d’Ornon Cedex, France |
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Abstract: | The milling behaviour of two naturally infected samples of durum wheat grain with contrasting levels of mycotoxins was studied. Although the two samples showed a similar milling behaviour, an increase of ~20% in deoxynivalenol (DON) levels was found in semolina from the sample containing the higher level of mycotoxin. However, even if the highest concentration of DON was found in fractions originating from the grain outer layers, the mycotoxin contamination in semolina and flours were not related to the amount of two compounds (ash or phytic acid) used to monitor these external tissues. The presence of the trichothecene-producing fungi in the inner-most semolina fraction was also shown using specific DNA primers and PCR amplification. Comparison of DON concentrations in the feed stock and corresponding output at each milling step or grinding of semolina fractions followed by sizing showed that concentration of mycotoxin occurs in the finest particles at the first processing steps. Therefore, DON contamination of milling fractions is not simply due to the presence of peripheral grain tissues. |
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Keywords: | deoxynivalenol (DON) durum wheat Fusarium spp milling mycotoxin Triticum |
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