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Chemical Profile and Biological Activities of Two Edible Plants: Chemical Investigation and Quantitative Analysis Using Liquid Chromatography Tandem Mass Spectrometry and Gas Chromatography Mass Spectrometry
Authors:Abdulselam Ertas  Mustafa Abdullah Yilmaz  Mehmet Boga  Nesrin Hasimi  Yeter Yesil  Ahmet C. Goren
Affiliation:1. Department of Pharmacognosy, Faculty of Pharmacy, Dicle University, Diyarbakir, Turkey;2. Dicle University Science and Technology Research and Application Center (DUBTAM), Dicle University, Diyarbakir, Turkey;3. Department of Pharmaceutical Technology, Faculty of Pharmacy, Dicle University, Diyarbakir, Turkey;4. Department of Nutrition and Dietetics, School of Health, Batman University, Batman, Turkey;5. Department of Pharmaceutical Botany, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey;6. TUBITAK UME, National Metrology Institute, Chemistry Group, Organic Chemistry Laboratory, Kocaeli, Turkey
Abstract:The objectives of this study were to define the phenolic and fatty acid profiles, anticholinesterase, antioxidant, antimicrobial activities, and total phenolic-flavonoid contents of Lycopsis orientalis and Tragopogon latifolius var. angustifolius which have been used as food source and food supplement in Anatolia and have never been examined before. Rosmarinic and quinic acids (21.11 and 11.46 mg g–1 extract, respectively) were found to be the most abundant constituents in L. orientalis and T. latifolius var. angustifolius among the studied 27 compounds by liquid chromatography tandem mass spectrometry. In the fatty acid compositions of L. orientalis and T. latifolius var. angustifolius that were determined by gas chromatography mass spectrometry, oleic (29.1%) and palmitic (28.7%) acids were identified as the major components, respectively. The high antioxidant activity of the methanol extract of L. orientalis shows parallelism to its rosmarinic acid content. Besides, this extract showed medium anticholinesterase activity. The results of the present study proves that the L. orientalis might also be used as a food source due to its high phenolic acid content and strong antioxidant property.
Keywords:Lycopsis orientalis  Tragopogon latifolius var. angustifolius  anticholinesterase  antimicrobial  antioxidant  LC-MS/MS
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