Inactivation of bacteriophages by thermal and high-pressure treatment |
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| Affiliation: | 1. Département de biochimie, microbiologie et bio-informatique, Faculté des sciences et de génie, Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Université Laval, Québec G1V 0A6, Canada;2. Centre de recherche de l''Institut universitaire de cardiologie et de pneumologie de Québec, 2725 chemin Sainte-Foy, Québec, QC G1V 4G5, Canada;1. School of Environment & Natural Resources, Renmin University of China, Beijing 100872, PR China;2. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, PR China;3. Xinjiang Key Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;1. University of Vienna, Department of Functional and Evolutionary Ecology, Division of Limnology, Djerassiplatz 1, 1030 Vienna, Austria;2. Helmholtz Zentrum München, Institute of Groundwater Ecology, Ingolstädter Landstraße 1, D-85764 Neuherberg, Germany;3. Helmholtz Zentrum München, Institute of Environmental Medicine, Neusaesser Strasse 47, 86165 Augsburg, Germany;4. German Environment Agency, Section II 3.1 “Protection of Drinking Water Resources”, Schichauweg 58, 12307 Berlin, Germany |
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| Abstract: | Dairy companies commonly experience fermentation failures due to bacteriophages that are spread mainly by milk, whey or air. Heat or high-pressure treatment may potentially reduce the phage titre, but further knowledge about the inactivation kinetics is desirable. Inactivation experiments were carried out with the commonly occurring lactococcal phages P001 and P008. Phage suspensions in calcium-enriched M17-broth were heated at 55–80 °C, or high-pressure treated at up to 600 MPa. Kinetic analysis showed that the order of inactivation reaction was above 1; thus, inactivation kinetics were approximated by a non-linear regression model. The Arrhenius parameters, rate constant, kp,T, and activation energy, EA (for heat treatments), and the volume of activation, ΔV# (for pressure treatments) were calculated. Both measured and calculated results indicate that phage P008 was the more heat- and pressure-resistant of the two. By combining the results from heat and pressure inactivations, a pressure–temperature diagram for phage P008 was established. |
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