| 气相色谱法测定配方奶粉中的脂肪酸和反式脂肪酸 |
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| 引用本文： | 刘冬豪.气相色谱法测定配方奶粉中的脂肪酸和反式脂肪酸[J].现代食品科技,2012,28(6):716-719. |
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| 作者姓名： | 刘冬豪 |
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| 作者单位： | 广州市食品工业卫生检测所,广东广州,510410 |
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| 摘 要： | 建立同时测定配方奶粉中脂肪酸和反式脂肪酸的气相色谱分析方法。样品中的脂肪酸和反式脂肪酸经乙酰氯-甲醇甲酯化处理后,用气相色谱仪测定,采用HP-88色谱柱分离定性,以十一碳酸甘油三酯为内标进行定量。结果表明:顺-反异构体脂肪酸分离效果较好,脂肪酸和反式脂肪酸的回收率在97.2%～104.6%之间,相对标准偏差小于3.5%,检出限为5μg/g。
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| 关 键 词： | 气相色谱 脂肪酸 反式脂肪酸 内标法 乙酰氯甲醇 |
| 收稿时间： | 2012/2/22 0:00:00 |
Determination of Cis and Trans Fatty Acids in Formula Powders by GC |
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| LIU Dong-hao.Determination of Cis and Trans Fatty Acids in Formula Powders by GC[J].Modern Food Science & Technology,2012,28(6):716-719. |
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| Authors: | LIU Dong-hao |
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| Affiliation: | LIU Dong-hao(Guangzhou Institute of Food Industry Hygiene Inspection,Guangzhou 510410,China) |
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| Abstract: | A internal standard method to determine cis-and trans-fatty acids in formula powders by gas chromatography was developed.Samples were methylesterized with acetyl chloride-MeOH,and analyzed by GC on a HP-88 column with FID as detector and triundecanoin as the internal standard.The results showed that cis-and trans-fatty acids in formula powders were well separated by this method.The recoveries,relative standard deviation and the detection limit of the GC method were 97.2%～104.6%,less than 3.5% and 5 μg/g,respectively. |
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| Keywords: | gas chromatography fatty acids trans fatty acids internal standard method acetyl chloride-MeOH |
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