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月饼中黄曲霉毒素B1检测前处理方法的改进
引用本文:李姣,佘之蕴,林耀文,刘海卿.月饼中黄曲霉毒素B1检测前处理方法的改进[J].现代食品科技,2012,28(3):364-366.
作者姓名:李姣  佘之蕴  林耀文  刘海卿
作者单位:广东产品质量监督检验研究院,广东顺德,528300
摘    要:通过对伍仁、蛋黄莲蓉、枣泥、水果、火腿、冰皮和吞拿鱼七种口味,共70批次月饼进行甲醇-水(1∶1,V/V)和三氯甲烷二次抽提两种处理方法对比,结果显示两种方法检测仅伍仁和吞拿鱼月饼差异显著,其余不显著.甲醇水提取方法检测结果重复性良好,加标回收率为84.0%114.7%,提高了月饼黄曲霉毒素B1的检测效率.

关 键 词:月饼  黄曲霉毒素B1  ELISA法
收稿时间:2011/12/15 0:00:00

Optimization of the Extraction Method of Aflatoxin B1 in Moon Cake
LI Jiao,SHE Zhi-yun,LIN Yao-wen and LIU Hai-qing.Optimization of the Extraction Method of Aflatoxin B1 in Moon Cake[J].Modern Food Science & Technology,2012,28(3):364-366.
Authors:LI Jiao  SHE Zhi-yun  LIN Yao-wen and LIU Hai-qing
Affiliation:(Guangdong Testing Institute for Product Quality Supervision,Shunde 528300,China)
Abstract:A study was conducted to optimize extraction method of Aflatoxin B1 from eight kinds of moon cake(mixed nuts,lotus seed with egg yolk,jujube paste,fruit,ham,snowy,and tuna) through ELISA.The detection results by two methods showed no significant difference except the detection in Mixed Nuts and Tuna.Better extraction efficacy was found using 50% methanol/water solution.The average recovery rate was 84.0%~114.7%.The method could improve the detection efficiency of aflatoxin B1 of moon cake.
Keywords:moon cake  aflatoxin B1  ELISA
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