| 胶体金免疫层析试纸条快速检测水和玉米中阿特拉津的残留 |
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| 引用本文： | 生威,那宇,张琳,刘冰,王硕.胶体金免疫层析试纸条快速检测水和玉米中阿特拉津的残留[J].现代食品科技,2012,28(3):360-363. |
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| 作者姓名： | 生威 那宇 张琳 刘冰 王硕 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院,教育部食品营养与安全重点实验室,天津300457 |
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| 摘 要： | 本文以胶体金作为抗体标记物,建立了一种检测阿特拉津残留的免疫层析试纸条快速检测方法.该方法的检出限为2ng/mL,10min内可以得到检测结果.本文以自来水和玉米作为样品进行了添加回收实验,水样不需要任何前处理直接检测,水样的检出限为2 ng/mL;玉米样品经简单前处理后检测,玉米样品的检出限为40 ng/g,且该方法的检测结果与间接竞争ELISA方法的结果一致.该方法具有成本低、操作简单、灵敏度高、检测快速,结果易于判定等优点,适用于大量样品的现场筛选.
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| 关 键 词： | 阿特拉津 胶体金 免疫层析试纸条 水 玉米 |
| 收稿时间： | 2011/12/22 0:00:00 |
Rapid Detection of Atrazine Residue in Water and Corn by Colloidal Gold-based Immunochromatographic Strip |
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| SHENG Wei,NA Yu,ZHANG Lin,LIU Bing and WANG Shuo.Rapid Detection of Atrazine Residue in Water and Corn by Colloidal Gold-based Immunochromatographic Strip[J].Modern Food Science & Technology,2012,28(3):360-363. |
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| Authors: | SHENG Wei NA Yu ZHANG Lin LIU Bing and WANG Shuo |
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| Affiliation: | (Key Laboratory of Food Nutrition and Safety,Ministry of Education of China,College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China) |
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| Abstract: | An immunochromatographic strip assay using colloidal gold-labeled antibody was developed.The visual limit of detection(LOD) of the assay for atrazine in PBS was 2 ng/mL.And the results could be obtained within 10 min.Spiked tap water sample was analyzed directly by the test strip without any pretreatment and the LOD was 2 ng/mL.Meanwhile,spiked corm sample was analyzed after simple pretreatment and the LOD was 40 ng/g.The results obtained by the immunochromatographic strip assay correlated well with those determine by indirect competitive ELISA.Because of its low cost,simple operation,high specificity,fast detection and easy-to-judgment results,the strip could serve as an on-site screening of a large number of samples. |
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| Keywords: | atrazine colloidal gold immunochromatographic strip water corn |
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