| 罗非鱼内脏蛋白酶特性及内脏蛋白自溶工艺研究 |
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| 引用本文： | 荣婧,蒋爱民,郭善广,吴著敏,吴德彬. 罗非鱼内脏蛋白酶特性及内脏蛋白自溶工艺研究[J]. 现代食品科技, 2012, 28(6): 651-654 |
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| 作者姓名： | 荣婧 蒋爱民 郭善广 吴著敏 吴德彬 |
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| 作者单位： | 华南农业大学食品学院,广东广州,510640 |
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| 基金项目： | 广东省科技计划项目，广东省重大科技专项 |
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| 摘 要： | 以罗非鱼内脏为原料,研究内源蛋白酶酶学特性；利用正交试验对其自溶工艺进行优化,结果表明:罗非鱼内脏内源蛋白酶在pH5～11范围内有较好的稳定性,在40～50℃条件下,罗非鱼内脏蛋白酶活性最稳定；罗非鱼内脏自溶的最佳工艺为:pH8,温度45℃,料液比1∶2条件下的自溶4h产物氯收率为73.21％,水解度为18.78％.
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| 关 键 词： | 罗非鱼内脏 内源蛋白酶 自溶 氮收率 水解度 |
| 收稿时间： | 2012-03-27 |
The Enzyme Characteristics of Endogenous Protease and Autolysis Technology of Tilapia Viscera |
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| RONG Jing,JIANG Ai-min,GUO Shan-guang,WU Zhu-min and WU De-bin. The Enzyme Characteristics of Endogenous Protease and Autolysis Technology of Tilapia Viscera[J]. Modern Food Science & Technology, 2012, 28(6): 651-654 |
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| Authors: | RONG Jing JIANG Ai-min GUO Shan-guang WU Zhu-min WU De-bin |
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| Affiliation: | (College of Food Science,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | The enzyme characteristics of endogenous protease were analyzed and the optimum autolysis conditions were determined by the orthogonal test.Result showed that the endogenous protease of Tilapia viscera was stable at pH of 5～11 and 40～50℃.The optimum conditions for autolysis of viscera were pH 8,temperature 45℃,raw/water materials 1:2 and reaction time 4 h,under which the nitrogen recovery ratio and degree of hydrolysis were 73.21% and 18.78% respectively. |
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| Keywords: | tilapia viscera endogenous protease autolysis nitrogen recovery ratio degree of hydrolysis |
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