恩诺沙星单克隆抗体的制备及酶联免疫分析方法的建立

采用抗恩诺沙星单克隆抗体建立酶联免疫吸附法（ELISA）用以检测恩诺沙星在动物源性食品中的残留。经方法学鉴定，本方法的检测范围为0．5～50μg／L，灵敏度为0．2μg／L，批内变异系数〈10％，批间变异系数〈20％。鸡肉、鱼肉、虾和蜂蜜样品的回收率分别为95．5％～107．5％、80．0％～101．3％、105．7％～122．7％和93．3％～111．3％。方法学鉴定结果符合免疫分析的基本要求。

Production of Monoclonal Antibodies Against Enrofloxacin and the Establishment of Enzyme-linked Immunosorbent Assay

Mice were immunized with ENRO-BSA conjugate, and seven stable cell lines excreting monoclonal antibodies against ENRO were obtained by hybridoma technique. Then ENRO19B10 was selected to develop an ELISA to analyze enrofloxacin residues in animal-based food products. The standard line of the assay was in the rang 0.5-50 ng/mL. The sensitivity was 0.2 μg/mL. The intra-and intro-assay CVs of 3 samples were lower than 10% and 20% respectively. The average recoveries of chicken muscle tissues, fish, shrimp and honey were in the range of 95.5 %~107.5 %，80.0 %~101.3 %，105.7 %~122.7 % and 93.3 %~111.3 % respectively.