HPLC法测定没食子酸甲酯含量的研究

采用HPLC方法进行没食子酸甲酯(MG)含量测定实验,UV特征吸收波长为279 nm。试验结果表明,色谱峰面积与试样溶液浓度的关系遵从比耳定律。运用统计学方法对测定值进行了分析研究,测量值数据的分布具有正态性;测量值的标准偏差S＝0.02%,符合B级精密度水平;两个实验室的测量值数据通过了F检验和t检验,无明显差异。由此表明,测量方法具有高精密度和准确度。该方法具有操作较为简便快捷、分析成本低廉、测定值稳定等特点。

Quantitative Determination of Methyl Gallate by HPLC Method

The content of methyl gallate (MG) was determined by HPLC. The characteristic absorption of MG was determined by ultraviolet scanning and the wavelength of characteristic absorption was 279 nm. The results showed that relationship between peak area of MG and the content of sample solutions in definite range obey Lombard Beer's law. The measured values were analyzed by statistical method, and it obey the normal distribution. The standard deviation of measured values was 0.02% which was within the limit of requirements. Statistic analysis showed that the measured values from two laboratories using F test and t test had no significant difference, which demonstrated that the method for determination of MG had high precision and accuracy. The method appeared to be simple and quick, cheap and stable.