Kinetic characterization and comparison of various protein crosslinking reagents for matrix modification |
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Authors: | Paul Slusarewicz Keng Zhu Tom Hedman |
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Affiliation: | 1. Orthopeutics, L.P., 111 Cooperative Way, Suite 210, Georgetown, TX, 78626, USA 2. Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA
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Abstract: | We have characterized the relative efficacies of a number of protein crosslinking agents that have the potential for use in the crosslinking of proteinaceous matrices both in vitro and in vivo. The crosslinkers tested were; l-threose (LT), Genipin (GP), Methylglyoxal (MG), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), proanthrocyanidin (PA) and glutaraldehyde (GA). The relative effectiveness of the crosslinkers with regard to their saturating concentrations was: GA > PA > EDC > MG = GP ? LT. Most of the crosslinkers displayed a pH dependence and were more effective at more alkaline pH. At optimal pH and saturating conditions, the relative reaction rates of the crosslinkers were: PA = GA > EDC > GP > MG ? LT. |
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