Effects of dietary saturated andtrans fatty acids on cholesteryl ester synthesis and hydrolysis in the testes of rats |
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Authors: | Takehiko Takatori Frederick Phillips Orville S Privett |
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Affiliation: | (1) The Hormel Institute, University of Minnesota, 55912 Austin, Minnesota;(2) Present address: Department of Legal Medicine, Hokkaido University Hospital, Hokkaido, Japan |
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Abstract: | Studies were made of the enzymic synthesis and hydrolysis of cholesteryl esters in rat testes. Weanling rats were fed for
14 weeks diets containing 5% by wt of hydrogenated coconut oil (HCO), a concentrate of ethyl elaidate and linolelaidate (TRANS),
devoid of essential fatty acids (EFA), or safflower oil (SAFF). Cholesterol esterifying activity was localized in the soluble
fraction, and cholesteryl ester hydrolase activity was distributed in both particulate and soluble fractions obtained from
tissue homogenates. The optimum pH was 6.0 for esterification and 6.9–7.0 for hydrolysis. Neither esterifying nor hydrolytic
activity was affected by freezing and thawing, but both reactions were inhibited by heat or sonication. The animals of both
the HCO and TRANS groups had developed an EFA deficiency before they were sacrificed. The EFA deficiency produced upon feeding
the HCO diet had no apparent effect on the synthesis and hydrolysis of cholesteryl esters in rat testes. The TRANS diet influenced
the development of the testes as judged by their size, and cholesterol esterifying and cholesteryl ester hydrolyzing activities
were suppressed in the testes of the animals of this group. A major difference in the effects of the HCO and TRANS diets on
the lipids of the testes was the relatively minor amount of eicosatrienoic acid (20∶3) and the elevated level of docosapentaenoic
acid (22∶5) in the cholesteryl esters of the testicular lipids of the TRANS group. |
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