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茶多酚与牛奶蛋白互作对蛋白质离体消化率的影响
引用本文:杜淑霞,欧仕益,贝惠玲,徐丽.茶多酚与牛奶蛋白互作对蛋白质离体消化率的影响[J].食品与发酵工业,2010(2).
作者姓名:杜淑霞  欧仕益  贝惠玲  徐丽
作者单位:广东轻工职业技术学院食品与生物工程系;暨南大学食品科学与工程系;
摘    要:采用胃蛋白酶体外消化法和HPLC法测定了茶多酚与牛奶蛋白互作后蛋白质的离体消化率和几种主要儿茶素含量的变化,并用酒石酸铁比色法测定了两者互作后不同结合状态茶多酚的含量。结果表明:在牛奶蛋白含量为0.5%、茶多酚含量在0-10.0 g/kg的模拟奶茶溶液中,蛋白质的离体消化率随茶多酚浓度的增大而下降,茶多酚含量≥2.0 g/kg时牛奶蛋白的离体消化率显著降低;等体积的纯牛奶和茶水同时食用时,茶水中茶多酚含量≥2.5 g/L时牛奶蛋白的离体消化率显著降低。实验结果还表明,食用条件下茶多酚与牛奶蛋白的结合以物理结合为主,而其中EGCG和ECG 2种儿茶素与牛奶蛋白有较强的反应活性,对牛奶蛋白的离体消化率起主要影响作用。

关 键 词:茶多酚  牛奶蛋白  互作  蛋白质离体消化率

Effects of Tea Polyphenols on in vitro Protein Digestibility in Milk Tea
Du Shuxia,Ou Shiyi,Bei Huiling,Xu Li.Effects of Tea Polyphenols on in vitro Protein Digestibility in Milk Tea[J].Food and Fermentation Industries,2010(2).
Authors:Du Shuxia    Ou Shiyi  Bei Huiling  Xu Li
Affiliation:Du Shuxia1,2,Ou Shiyi2,Bei Huiling1,Xu Li1 1(Department of Food Bioengineering,Guangdong Industry Technical College,Guangzhou 510300,China)2(Department of Food Science , Engineering,Jinan University,Guangzhou 510632,China)
Abstract:Changes in vitro protein digestibility and contents of catchins in the system of tea polyphenol with milk protein was investigated.The results showed that in the model milk tea which contains 0.5 % milk protein and 0-10.0 g/kg tea polyphenols,the in vitro digestibility of the protein decreased as tea polyphenols content increased.It decreased significantly when the contents of tea polyphenols was more than 2.0 g/kg.When milk and tea mixed together at the same ratio and polyphenols was more than 2.5 g/L,the ...
Keywords:tea polyphenols  milk protein  interaction  digestibility in vitro of protein  
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