Self-contained, biomolecular motor-driven protein sorting and concentrating in an ultrasensitive microfluidic chip |
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Authors: | Lin Chih-Ting Kao Ming-Tse Kurabayashi Katsuo Meyhofer Edgar |
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Affiliation: | Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA. |
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Abstract: | We developed a molecular sorter that operates without external power or control by integrating the microtubule-based, biological motor kinesin into a microfluidic channel network to sort, transport, and concentrate molecules. In our devices, functionalized microtubules that capture analyte molecules are steered along kinesin-coated microchannel tracks toward a collector structure, concentrated, and trapped. Using fluorescent analyte molecules and nanoliter sample volumes, we demonstrated 14 fM sensitivity, even in the presence of high concentrations of other proteins. |
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