用于酶热传感器的酶标甲胺磷农药的低温贮存研究

采用加速实验中获得的较优保护剂配方(2.0mol/L氯化钠、4.0mol/L木糖醇、1.25mol/L甘油),对酶热传感器中酶标甲胺磷农药低温贮存过程中的酶活变化进行了研究。结果显示:加或未加复合保护剂时,酶标农药的贮存温度与酶活损失速率常数之间符合Arrhenius关系,且酶标农药-18℃条件下贮存6个月后,酶活保留率分别为77.13%(未加保护剂)和97.44%(加保护剂)。与化学动力学方法获得的理论预测值相比,相对标准偏差分别为3.10%(未加保护剂)和0.51%(加保护剂)。这一结果将为下一步的农残检测酶热传感器的开发奠定基础。

Research of Enzyme-labeled Methamidophos Cryopreservation for Enzyme Thermal Sensor

Cryopreservation of enzyme-labeled methamidophos was studied using the composite protective agent(2.0mol /L NaCl,4.0mol/L xylitol and 1.25mol/L glycerol) obtained from accelerated test.The results show that the relationship between enzyme activity loss rate and storage temperature meet arrhenius equation.After 6 months,the retention rate of enzyme activity is 77.13%(without protective agents) and 97.44%(with protective agents) under the storage conditions of-18℃.Comparing to theoretical prediction value according to chemical kinetics,the relative standard deviation was 3.10%(without protective agents) and 0.51%(with protective agents),respectively.The result will lay the foundation for the development of thermal pesticide residue biosensor.