酶联免疫吸附法快速测定不同样品 基质中三聚氰胺

目的 探求不同样品基质中三聚氰胺残留量的快速检测方法.为快速筛查不同种类食品中非法添加三聚氰胺提供技术保障.方法 采用酶联免疫吸附法在奶制品(奶粉、液态奶)、成品饲料(鸡饲料、猪饲料)、饲料原料(鱼粉、肉骨粉、豆粕、麸皮)、肉类(鸡肉、猪肉、内脏)等样品基质中添加一定浓度的三聚氰胺进行测定,并对检测结果进行分析.结果 酶联免疫试剂盒对奶制品和肉类检出限均能达到1.0 mg/kg;对成品饲料基质中的三聚氰胺的检测,检出限可达到2 mg/kg,而对饲料原料中的三聚氰胺的检测,检出限都不能达到2 mg/kg.结论 对奶制品中的三聚氰胺检测完全符合我国的临时限量标准;对成品饲料中的三聚氰胺残留的检测同样符合其限量标准(2.5 mg/kg),而对饲料原料中的三聚氰胺的检测,由于不同基质中检出限不同,不能直接采用酶联免疫法进行快速筛查;酶联免疫法也适用于肉类中的三聚氰胺的检测.

Rapid Detection of Melamine in Different Matrixes of Food Samples by ELISA

Objective To investigate the method of rapid screening melamine in different matrixes of food samples, and to provide technical support for the detection of melamine unlawfully added to foods. Method Adding melamine to different matrixes of food samples such as dairy products (milk powder, liquid milk), animal feeds (chicken feed, pig feed), raw material of feeds (fish meal, meat and bone meal, soybean meal, wheat bran), meat (chicken, pork, organ meat), and detecting the melamine with enzyme-linked immunosorbent assay (ELISA). Results The detectable limits of melamine for dairy products and meat were lower than 1.0 mg/kg; the detectable limits for feeds were lower than 2.0 mg/kg, whereas the detectable limits for raw materials of feeds were more than 2.0 mg/kg. Conclusion The detection results of melamine in dairy products was fully conformed with China''s temporary limited standard (1.0 mg/kg); the results of melamine in feeds were also suited to the limited standards (2.5 mg/kg). However, the ELISA method used for screening melamine in raw materials of feeds should be further improved. ELISA method can also be applicable to the detection of melamine in meat.