Influence of storage time on bond strength of self-etching adhesive systems to artificially demineralized dentin after a papain gel chemical–mechanical agent application |
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| Affiliation: | 1. School of Dentistry and Research Institute, São Leopoldo Mandic, Campinas, São Paulo, Brazil;1. Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Universidad de Málaga, Spain;2. Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain;3. CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Malaga, Spain;4. Unidad de Gestión Clínica de Cirugía General, Digestiva y Transplantes, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Málaga, Spain;5. Unidad de Gestión Clínica de Cirugía General, Digestiva y Transplantes, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain;6. CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Málaga, Spain;7. Unidad de Gestión Clínica de Aparato Digestivo, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Málaga, Spain;1. Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Gwangju, Korea;2. Department of Physiology, School of Dentistry, Dental Science Research Institute, Gwangju, Korea;3. Department of Pharmacology and Dental Therapeutics, School of Dentistry, Dental Science Research Institute, Gwangju, Korea;4. Research Center for Biomineralization Disorders, Chonnam National University, Gwangju, Korea;1. Department of Stomatology (Endodontics Section), University of Sevilla, Sevilla, Spain;3. Department of Normal and Pathologic Cytology and Histology, University of Sevilla, Sevilla, Spain;2. Department of Medical Biochemistry and Molecular Biology, Virgen Macarena University Hospital, Sevilla, Spain;1. Department of Periodontology, Faculty of Dentistry, Ankara University, Ankara, Turkey;2. Molecular Biology Laboratory, Faculty of Dentistry, Ankara University, Ankara, Turkey;3. Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey;4. Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Ankara University, Ankara, Turkey;1. Center for Hypothalamic Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA;2. Division of Endocrinology & Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA;3. Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, TX, USA;4. Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA;1. Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;2. Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;3. Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;4. Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;5. Department of Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;6. International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba 305-8575, Japan;7. Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M University Health Sciences Center, Dallas, TX 75246, USA |
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| Abstract: | ObjectivesThe aim of this study was to evaluate the influence of storage time on the bond strength of self-etching adhesive systems to artificially demineralized dentin submitted to application of a papain-based chemical mechanical agent for carious tissue removal.Materials and MethodsTwenty-four blocks of human coronal dentin were randomly divided into 2 groups: (1) restored with a two-step self-etching adhesive system (Clearfil SE Bond); (2) restored with a one-step self-etching adhesive system (One-Up Bond F Plus). After artificial caries induction, the specimens were treated with papain-based gel, received an application of the adhesive system according to the respective group, and blocks of microhybrid resin composite measuring 5.0 mm high and 5.0 mm wide were fabricated incrementally on the tooth, which would later be cut to obtain sticks with a bond area of around 1 mm2, for use in the microtensile tests. After this, half of the sticks obtained from each tooth were submitted to the microtensile test 24 h later, while the other half were stored in water, in an oven at 37° C for a period of 180 d. The specimens were submitted to the microtensile test in a Universal Test Machine at a speed of 0.5 mm/min. The surfaces of the fractured test specimens were examined visually under a stereoscopic loupe in order to classify the fracture type. After exploratory analysis of the data, the two-way Analysis of Variance (ANOVA) and the Tukey test were applied. The data on the fracture types were analyzed by the Exact Fisher test.ResultsNo statistically relevant differences were verified among the means of the microtensile bond strength values of the adhesive systems evaluated in the different storage times. Mixed and adhesive fractures types were observed for both groups.ConclusionStorage time did not influence the bond strength of the two-step or one-step self-etching adhesive systems to artificially demineralized dentin submitted to application of a papain-based chemical mechanical agent for carious tissue removal. Both adhesive systems provided similar bond strength at different storage times. |
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