发酵优化制备L-赖氨酸盐酸盐-13C6、15N2

采用北京棒杆菌（Corynebacterium pekinense）ZLD118（HS^-、AEC^r）发酵制备稳定同位素双标记的L-赖氨酸盐酸盐-¹³C₆、¹⁵N₂。对菌种的发酵培养条件和发酵培养基配方进行优化，采用优化后的发酵工艺制备高丰度稳定同位素¹³C、¹⁵N双标记L-赖氨酸，经离子交换树脂提纯、活性炭脱色精制后，产品的¹³C丰度达98.61%，¹⁵N丰度为98.15%，纯度为98.47%，产率为88.8%。结果表明，该工艺可进一步用于放大制备。

Preparation of L-Lysine hydrochloride-13C6,15N2 by Optimized Fermentation

Stable isotope double labeledL-Lysine hydrochloride ¹³C₆、¹⁵N₂ was preparaed by Corynebacterium pekinense ZLD118（HS^-、AEC^r）through fermentation. The conditions of microbial fermentation and the formula of fermentation medium had been optimized. The fermentation ofL-Lysine labeled with high abundance stable isotopoe¹³C and¹⁵N was successfully performed with the optimized process. The high abundanceL-Lysine fermentation broth was purified by ion-exchange resin ，refined after decolorization by activated carbon and the final productL-Lysine hydrochloride ¹³C₆、¹⁵N₂ was got. The¹³C abundance of the product was 98.61% and the¹⁵N abundance was 98.15%，its purity was 98.47%. The yield ofL-Lysine hydrochloride ¹³C₆、¹⁵N₂ reached 88.8%. The results showed that this process can be further used for preparation in up scale.