模拟胃肠道反应器的参数建立与冷模实验研究

体外消化和发酵实验紧密结合才能模拟完整消化道的功能,现有的模拟胃肠消化和大肠发酵的反应器结构各异且不通用,组合多个独立反应器则增加了实验的复杂程度和设备成本。集成了两类反应器的优势特征,开发了一种采用可编程逻辑控制器（PLC）的模拟胃肠道反应器（GSR）,模拟了整体的胃肠道动态特征并与体内数据进行了比较。结果表明模拟胃肠道反应器既可准确模拟胃和小肠的蠕动、排空和混合等动态特征,又可提供肠道微生物的发酵环境。尾气排放和收集装置的设计实现了尾气快速排出和尾气体积的准确计量。模拟胃肠道反应器可为体外探索益生元对肠道微生物作用等研究提供帮助。

Parameter estimation and cold model experiments of gastrointestinal simulation reactor

In vitro digestion and fermentation experiments should be closely combined to simulate the function of the intact gastrointestinal tract. However, the existing simulated reactors for gastrointestinal digestion and large intestinal fermentation have different structures and are not universal. Combining the separated reactors would increase the complexity and the cost of the experiments. Integrated the dominant features of both types of reactors, a gastrointestinal simulation reactor(GSR) using a programmable logic controller(PLC) was developed in the present work. The dynamic characteristics of overall gastrointestinal tract were simulated and compared to the data in vivo. It shows that the developed reactor can accurately simulate the dynamic characteristics of peristalsis, emptying and mixing in the stomach and small intestine, and provide a fermentation environment for intestinal microbes. The exhaust gas discharge and collection device was considerately designed, the exhaust gas was quickly discharged and the volume could be accurately measured. The gastrointestinal simulation reactor can help to explore the role of prebiotics in intestinal microbes in vitro.