The method of surface PEGylation influences leukocyte adhesion and activation |
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Authors: | Z Ademovic B Holst R A Kahn I Jørring T Brevig J Wei X Hou B Winter-Jensen P Kingshott |
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Affiliation: | 1. Danish Polymer Centre, Ris? National Laboratory, Frederiksborgvej 399, 4000, Roskilde, Denmark 2. Bioneer A/S, Mammalian Cell Biology, Kogle Alle 2, 2970, H?rsholm, Denmark 3. Nuclear Research Department, Ris? National Laboratory, Frederiksborgvej 399, 4000, Roskilde, Denmark
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Abstract: | The influence of different surface modifications with poly(ethyleneglycol) (PEG) layers on the adsorption of fibrinogen and
the adhesion and activation of macrophage-like human leukocytes was investigated. Poly(ethylene terephthalate) (PET) was modified
using pulsed AC plasma polymerization with two types of starting monomers to generate: 1) a reactive acid surface using maleic
anhydride (MAH) as monomer, and 2) a PEG-like surface using diethyleneglycol methyl vinyl ether (DEGVE) as monomer. The MAH
surface was used as a reactive platform to graft linear chains of non-fouling mPEG via an intermediate layer of poly(ethyleneimine)
(PEI) under lower critical solution temperature (LCST) conditions of the mPEG. The DEGVE monomer is used to create PEG-like
layers by use of low power plasma conditions. The ability of the surfaces to resist protein adsorption was investigated quantitatively
using 125I-radiolabeled human fibrinogen, and the conformation of the adsorbed protein was tested using an anti-fibrinogen monoclonal
antibody in an enzyme-linked immunosorbent assay. The results showed that PEGylated surfaces adsorbed significantly less (up
to 90% less) fibrinogen, and that unfolding of adsorbed fibrinogen was more pronounced on the linear mPEG layers than on the
PEG-like plasma polymer surfaces. Adhesion of in-vitro differentiated macrophage-like U937 cells was reduced on both the PEG-like
plasma polymer surfaces and the linear mPEG layers compared to the unmodified PET surface, but cells adhering to the PEG-like
plasma polymer surfaces secreted less tumor necrosis factor-α (TNF- α) than cells adhering to the linear mPEG layers. In conclusion,
the method for preparing non-fouling surfaces for long-term implanted devices influence surface-induced cellular responses
of the host. |
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