Specific response of osteoblast-like cells on hydroxyapatite layer containing serum protein |
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Authors: | Takashi Kizuki Masataka Ohgaki Shizuko Ichinose Satoshi Nakamura Kazuaki Hashimoto Yoshitomo Toda Yoshiyuki Yokogawa Kimihiro Yamashita |
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Affiliation: | (1) Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology, 2266-98 Anagahora, Shimoshidami, Moriyama-ku Nagoya, 463-8560, Japan;(2) Institute of Biomaterials and Bioengineering Tokyo Medical and Dental Univ., 2-3-10, Kandasurugadai, Chiyoda Tokyo, 101-0062, Japan;(3) Instrumental Analysis Research Center, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo Tokyo, 113-8510, Japan;(4) Industrial Inorganic Chemistry, Graduate School of Engineering, Chiba Institute of Technology, 2-17-1, Tsudanuma, Narashino City Chiba, 275-0016, Japan |
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Abstract: | Accelerations of bone-like apatite deposition and cell growth on an electrically polarized ceramic hydroxyapatite have been
reported. A relationship between these phenomena was investigated in a previous report, and then it was suggested that osteoblast-like
cell’s (MC3T3-E1) growth had relevance to the mineral growth. The effect of the formed apatite layer especially appeared to
be on the cell adhesion. The acceleration of cell proliferation on the polarized HAp has been shown using fibroblastic cell
(L929) and nerve cell (SK-N-SH) lines, therefore the effect of the layer on L929 and SK-N-SH was investigated to support the
mechanism of acceleration of cell proliferation by polarization of HAp. In this study, the effect of the bone-like apatite
layer was not confirmed on L929 cell’s growth. On the other hand, the acceleration of nerve cell’s proliferation was confirmed
on the formed apatite layer. However, the remarkable improvement of the cell adhesion of SK-N-SH was not confirmed on the
apatite layer. Consequently, it was considered that the bone-like apatite containing serum protein obtained by the coprecipitation
of bone-like apatite and serum protein has a pronounced role only in the activity of osteoblast-like cells. |
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