Platelet-activating factor stimulates expression of IL-1 beta mRNA in THP-1 cells |
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| Authors: | Roger A. Barthelson Frank H. Valone |
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| Affiliation: | (1) Department of Medicine, University of California San Francisco, 111H, 4150 Clement Street, 94121-1598 San Francisco, California;(2) the Veterans Administration Medical Center, 94121-1598 San Francisco, California |
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| Abstract: | A human monocytic cell line (THP-1) was used to study the effects of PAF (platelet-activating factor) on the expression of IL-1 beta mRNA. THP-1 cells were incubated with 10 pM PAF in the presence or absence of 0.1 μg/mL endotoxin for 4 hr, after which cytoplasmic RNA was extracted and subjected to Northern hybridizations. PAF, alone and in combination with endotoxin, caused an increase in mRNA levels for IL-1 beta. The magnitude of the effects of PAF on IL-1 beta mRNA levels matched closely the effects seen at the level of protein synthesis, suggesting that the effects of PAF on IL-1 beta release may result largely from its effects on IL-1 beta mRNA levels. |
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