Optimization of carotenoids extraction from Rhodobacter sphaeroides |
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| Authors: | Zhenxin Gu Chen Deming Chen Zhigang |
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| Affiliation: | a College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
b College of Foreign Language Studies, Nanjing Agricultural University, Nanjing 210095, People's Republic of China |
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| Abstract: | The comparison of the three methods ultrasonic assisting, grinding and HCl assisting on carotenoids extraction yield from Rhodobacter sphaeroides (R. sphaeroides) was carried out. The HCl-assisted extraction was found to be the most effective. And then, based on HCl-assisted extraction method, the effect of temperature, solvent-solid ratio and duration on the yield of carotenoids extracted from R. sphaeroides was investigated using single factor and Box-Behnken experimental design. The optimal conditions for HCl-assisted extraction of carotenoids from R. sphaeroides were found to be: temperature 30 °C, solvent-solid ratio 40 ml/g, duration 40 min. The first two extractions were enough to approximately exhaust the entire content of carotenoids in R. sphaeroides. |
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| Keywords: | Rhodobacter sphaeroides Carotenoids Optimization Extraction Box-Behnken design |
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