| PVY、CMV双价抗性RNA沉默表达载体的构建 |
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| 引用本文： | 王凤龙,吴元华.PVY、CMV双价抗性RNA沉默表达载体的构建[J].烟草科技,2007(12):55-59. |
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| 作者姓名： | 王凤龙 吴元华 |
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| 作者单位： | 1. 沈阳农业大学,沈阳市东陵区马官桥,110161;中国农业科学院烟草研究所,青岛市崂山区科苑经四路11号,266101
2. 沈阳农业大学,沈阳市东陵区马官桥,110161 |
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| 基金项目： | 国家烟草专卖局资助项目 |
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| 摘 要： | 采用RT-PCR方法克隆了PVYCP基因、PVYHC-Pro基因、CMVCP基因和CMV2b基因的保守片段,分别将PVYCP片段和CMV2b片段、PVYHC-Pro片段和CMV2b片段、PVYHC-Pro片段和CMVCP片段连接起来,以此为靶标构建并得到3个RNA沉默表达载体pCAMPb、pCAMHb和pCAMHP。获得的载体可以应用于植物转基因抗病毒育种工程中。
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| 关 键 词： | 片段克隆 载体构建 RNA沉默 双价抗性 |
| 文章编号： | 1002-0861(2007)12-0055-05 |
| 修稿时间： | 2007年8月13日 |
Construction of PVY and CMV Binary Virus Resistant RNA Silencing Vector |
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| WANG FENG-LONG,WU YUAN-HUA.Construction of PVY and CMV Binary Virus Resistant RNA Silencing Vector[J].Tobacco Science & Technology,2007(12):55-59. |
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| Authors: | WANG FENG-LONG WU YUAN-HUA |
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| Abstract: | Using RT-PCR method, the conserved fragment of PVY CP gene, PVY HC-Pro gene, CMV CP gene and CMV 2b gene were cloned, then the fragments of PVY CP and CMV 2b, PVY HC-Pro and CMV 2b, PVY HC-Pro and CMV CP were connected respectively. Three RNA silencing vectors, pCAMPb, pCAMHb and pCAMHP, were constructed using the connected fragments as the target, and the constructed vectors could be used in plant transgenic virus resistance breeding. |
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| Keywords: | Fragment clone Vector construction RNA silencing Binary virus resistance |
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