| 真菌纤维素酶系催化纤维素水解活力的测定方法简介 |
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| 引用本文： | 武彬,赵越,高培基.真菌纤维素酶系催化纤维素水解活力的测定方法简介[J].纤维素科学与技术,2007,15(1):50-51. |
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| 作者姓名： | 武彬 赵越 高培基 |
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| 作者单位： | 山东大学,微生物技术国家重点实验室,山东,济南,250100 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);山东省自然科学基金 |
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| 摘 要： | <正>在测定纤维素粗酶样品对不溶性固体纤维素的水解活力时,尽管选用同一纤维素底物,并以同样的纤维素粗酶样品在相同温度下水解,测定结果受反应条件如纤维素底物浓度、纤维素酶浓度以及水解时间等因素的影响而相互差别很大。而纤维素粗酶作为多个纤维素酶组分的混合物,其糖化能力更加能够反应其中各个组分之间的协同水解转化能力。本文中选择了比底物水解率(SSC)作为纤维素酶浓度的函数,即单位纤维素酶每分钟对滤纸的水解百分比作为纤维素粗酶样品不同浓度的目的函数,从而克服了以上条件对纤维素酶活测定的影响。并以水解过程中SSC瞬时速率的AUC(Area under curve)对加入纤维素酶的量做图得到的斜率评价纤维素酶样品的水解能力。经检验,该方法也适用于以棉纤维、微晶纤维素PH101和磷酸膨胀纤维素等不同纤维材料为底物时纤维素酶粗酶样品糖化能力的测定。
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| 关 键 词： | 纤维素 纤维素酶 转化 水解 |
| 文章编号： | 1004-8405(2007)01-0050-02 |
| 修稿时间： | 2006年9月25日 |
A New Approach to Measurement of Saccharifying Capacities of Crude Cellulase |
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| WU Bin,ZHAO Yue,GAO Pei-ji.A New Approach to Measurement of Saccharifying Capacities of Crude Cellulase[J].Journal of Cellulose Science and Technology,2007,15(1):50-51. |
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| Authors: | WU Bin ZHAO Yue GAO Pei-ji |
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| Abstract: | A practical,quantitative approach has been designed,which makes it possible to accurately estimate the saccharifying activities of crude cellulase preparations for insoluble cellulosics.The challenge in activity determination imposed by changes in hydrolysis time and concentration of cellulase and cellulosics on the assay could be overcome by selection of the specific conversion percentage of cellulose as a function of cellulase concentration,that is,the hydrolysis percentage of filter paper by unit cellulase per minute,as the objective function with respect to different concentrations of crude cellulase.A rational and governing equation for crude cellulase assay was derived,and reliable results for quantitatively estimating the saccharifying activities of crude cellulases during the progress of hydrolysis of several cellulosic substrates were obtained. |
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| Keywords: | cellulase cellulose conversion hydrolysis |
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