微波辅助法提取马尾松树皮总黄酮的研究

以水为溶剂采用微波辅助辐射法提取了马尾松树皮总黄酮。以芦丁标准品为对照品，用分光光度法测定马尾松树皮总黄酮的含量；探讨了料液比、微波辐射功率、微波辐射时间、浸提时间、浸提温度等因素对马尾松树皮总黄酮提取率的影响，通过正交实验得出微波辅助提取马尾松树皮总黄酮的最佳条件为：料液比1：25（g：mL）、微波辐射功率350W、微波辐射时间3×40s（间歇3次）、浸提时间4h、浸提温度60℃，在此条件下，总黄酮提取率达到3．08％。微波辅助下以水为溶剂提取马尾松树皮总黄酮高效、快速、经济，具有较好的实用价值。

Microwave-Assisted Extraction of Total Flavonoids in Pinus Massoniana Bark

Total favonoids in Pinus massoniana bark was extracted by microwave-assisted extraction using water as solvent. With rutin standard as reference substance,flavonoids content in Pinus rnassoniana bark was determined by spectrophotometry. The effects of solid-liquid ratio, microwave irradiation power, microwave ir- radiation time,soaking time, soaking temperature on extraction rate of total flavonoids in Pinus massoniana bark were studied. The optimum conditions of microwave-assisted extraction of total flavonoids in Pinus massoniana bark were obtained through single factor experiment and orthogonal experiment as follows：solid-liquid ratio of 1 ： 25 （g ： mL）, microwave irradiation power＇of 350 W, microwave irradiation time of 3 X 40 s（intermit- tent 3 times） , soaking time of 4 h, soaking temperature of 60 ℃. Under above eonditions, the extraction rate could reach 3.08 %. Microwave-assisted extraction of total flavonoids in Pinus rnassonlana bark with water as solvent was rapid, efficient and economic.