Adriana and Luisa Castellucci Award Lecture 1997. Placental pathology in obstetrics: adaptation or failure of the villous tree?

The biochemical and ultracytochemical localization of alkaline phosphatases in permeabilized cells of Saccharomyces cerevisiae 257 has been studied. The treatment with non-ionic surfactant Triton X-100 allows the penetration of the substrate into intact yeast cells and thus provides detailed detection of the enzyme activity in ultracytochemical studies.