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Cultivation of anaerobic granular sludge in UASB reactors with aerobic activated sludge as seed
Affiliation:1. Department of Environmental Engineering, Tsinghua University, Beijing, People''s Republic of China;2. Chengdu Biogas Research Institute of Agriculture Ministry of China, Chengdu, Sichuan, People''s Republic of China;3. Changzhou Environmental Pollution Monitoring Centre, Changzhou, Jiangsu, People''s Republic of China;1. Department of Civil and Environmental Engineering, University of Alberta, Donadeo Innovation Centre for Engineering, Edmonton, Alberta, T6G 1H9, Canada;2. Sub-department of Environmental Technology, Wageningen University, P.O. Box 17, 6700 AA, Wageningen, The Netherlands;1. School of Civil Engineering, Beijing Jiaotong University, Beijing 100044, PR China;2. School of Civil Engineering and Architecture, East China Jiaotong University, Nanchang 330013, PR China;3. Department of Civil & Environmental Engineering, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore;4. Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, Beijing 100044, PR China;1. Center of Analysis and Measurement, Fudan University, Shanghai, 200433, China;2. Department of Environmental Science and Engineering, Fudan University, 2005 Songhu Road, Yangpu District, 200438, China;3. Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan;4. Department of Civil Engineering, University of Calgary, Calgary, Canada;1. Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Jinjing Road 26#, Tianjin 300384, PR China;2. Department of Building, Civil, and Environmental Engineering, Concordia University, 1455 de Maisonneuve Blvd. W., Montreal, Quebec, Canada
Abstract:Methanogenic bacteria of 108 g SS−1 in the activated sludges from an aeration tank treating sewage and from a secondary sedimentation tank of an activated sludge plant treating textile dyeing wastewater were enumerated by the Most Probable Number (MPN) technique. By using the two activated sludges as the seed material, anaerobic granular sludges were obtained at 35°C in two lab-UASB reactors having volumes of 29 and 481, and treating a glucose molasses solution of 1000–3500 mg COD 1−1 and citrate wastewater of 20,000–36,000 mg COD 1−1 respectively. The characteristics of granulation using the activated sludge as the seed were similar to those using digested sewage sludge as the seed. It is shown that activated sludge is readily available seed material for an anaerobic reactor. The growth of methanogenic bacteria in the activated sludge can be attributed to the existence of some anaerobic nuclei in the activated sludge flocs. The factors for the cultivation of granular sludge by using the activated sludge are also discussed.
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