Au@Ag纳米粒子表面增强拉曼光谱法高灵敏检测孔雀石绿

采用晶种增长-抗坏血酸室温还原法有效合成了Au@Ag纳米粒子。通过进一步优化Au@Ag纳米粒子的组成和体积、助剂H（NaCl和KBr的混合溶液）的体积以及待测样品的加样顺序，在便携式拉曼光谱仪上成功实现了孔雀石绿（MG）的快速高灵敏检测。其对MG检测线性范围为0.5 μg/L~10 μg/L，线性相关系数达到0.991，实际可测最低浓度为0.5 μg/L。将本方法用于基围虾虾肉中MG的检测，直接检测未检出；用前处理液配制MG模拟样本，10 μg/L时信号明显可见。本方法有望用于水产品中MG的快速高灵敏检测。

Highly Sensitive Detection of Malachite Green by Surface-Enhanced Raman Scattering Method Using Au@Ag Nanoparticles

Au@Ag nanoparticles with excellent surface enhancement were synthesized by a seeded growth-ascorbic acid reduction. Through optimizing the molar ratio of Ag/Au， the volume of Au@Ag nanoparticles，the volume of auxiliary H （the mixture of KBr and NaCl） and the adding order of auxiliary H and tested samples，a highly sensitive and rapid qualitative detection of malachite green （MG） was achieved with a portable Raman spectrometer， whose detection limit was 0.5 μg/L with a linear range from 0.5 μg/L to 10 μg/L （R2 =0.991）. This detection method was also applied in the determination of MG in a shrimp sample from Baishazhou market of Wuhan， and no MG was found. However，the solution containing 10 μg/L MG were detected with strong signals. This detection method is expected to be used in detecting MG for the aquatic products.