MCR-ALS与HPLC-DAD相结合同时测定喜树碱及其衍生物

将多元曲线分辨-交替最小二乘(MCR-ALS)与高效液相色谱-二极管阵列检测器(HPLC-DAD)检测相结合,对伊立替康、7-乙基-10-羟基喜树碱和喜树碱的色谱重叠峰进行分辨。采用MCR-ALS同时分析多个数据矩阵组成的方式,以克服MCR-ALS在单一重叠峰数据矩阵的解析中遇到的缺秩问题。讨论了影响分辨结果的几种因素,包括被分析物之间的光谱相似度、色谱峰的分离度和干扰物浓度等。分辨所得光谱还原率高,定量结果可靠,说明MCR-ALS能有效地解决喜树碱及其衍生物色谱分析中重叠峰的二阶校正问题。

Simultaneous determination of camptothecin and its derivants using MCR-ALS with HPLC-DAD

Discussed in this paper is the application of multivariate curve resolution-alternating least squares(MCB-ALS)with high performance liquid chromatography-diode array detection(HPLC-DAD)on the resolution of overlapping chromatographic peaks of irino- tecan(CPT-11),7-ethyl-10-hydroxyl-camptothecin(SN-38)and camptothecin(CPT,as intruder).Because of spectra similarity and serious overlapping,MCR-ALS was used when several matrices were analyzed simultaneously and in this case the rank-deficiency could be overcome.Some factors that affected resolution when MCR-ALS was applied to augmented matrix were discussed,including spectra similarities of components,type of overlapping peaks and concentration of intruder.The results obtained showed that the resolved spec- tra coincided perfectly with real spectra and the resolved calculated concentrations were precision.MCR-ALS was successful to solve resolution of chromatographic overlapping peaks of Camptothecin and its Derivants.