| 箱式固态发酵生产Bt生物农药的研究 |
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| 引用本文： | 李成,弓爱君,邱丽娜,苑海涛.箱式固态发酵生产Bt生物农药的研究[J].化学与生物工程,2009,26(4). |
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| 作者姓名： | 李成 弓爱君 邱丽娜 苑海涛 |
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| 作者单位： | 北京科技大学应用科学学院生物科学与技术系,北京,100083 |
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| 基金项目： | 国家标准物质资源共享平台建设项目 |
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| 摘 要： | 利用自制箱式固态发酵设备,以价格低廉的麸皮等农业下脚料为主要培养基,对Bt HD-1菌种公斤级固态发酵扩大条件进行了优化研究.确定最佳发酵条件为:在种龄8 h时接种,含水量为55%,pH=7.5,箱体内培养基厚度控制在4 cm,接种量为15%,发酵时间为45 h.在此条件下,单次发酵量达到1.25 kg,毒力效价稳定在13 000 IU·mg-1以上.实现了Bt生物农药公斤级固态发酵的生产,为Bt生物农药的固态发酵大规模工业化生产奠定了基础.
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| 关 键 词： | 生物农药 苏云金芽孢杆菌 固态发酵 |
Study on Production of Bt Biopesticide by Box-Type Solid-State Fermentation |
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| LI Chenɡ,GONG Ai-jun,QIU Li-na,YUAN Hai-tao.Study on Production of Bt Biopesticide by Box-Type Solid-State Fermentation[J].Chemistry & Bioengineering,2009,26(4). |
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| Authors: | LI Chenɡ GONG Ai-jun QIU Li-na YUAN Hai-tao |
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| Affiliation: | Department of Biological Science and Technology;School of Applied Science;University of Science and Technology Beijing;Beijing 100083;China |
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| Abstract: | In this paper,scale of kg class production of Bt biopesticide was studied on wheat bran based media using the self-made box-type solid-state fermentation equipment.The result showed that,the most amount of crystal protein was obtained under the following conditions:seed age 8 h,moisture content 55%,pH value 7.5,inoculum amount 15%,fermentation time 45 h,the thickness of medium 4 cm.Under above conditions,the protein amount could be 1.25 kg for a single fermentation process,and the bioassay efficacy of the p... |
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| Keywords: | biopesticide Bacillus thuringiensis solid-state fermentation |
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