高效液相色谱法测定1,15-十五碳二元酸的方法研究

采用液相色谱内标法建立了环十五内酯合成中的重要化合物1,15-十五碳二元酸的定量分析方法。通过实验确定了最优色谱条件：色谱柱C18（4.6×250mm）,以月桂酸为内标物,流动相为甲醇∶乙腈∶水=80∶5∶15（0.1%三氟乙酸）,流速为1.2mL/min,检测波长为210nm。方法的相关性好（γ〉0.9990）,样品测定的相对标准偏差RSD〈1%,加标回收率为99.25%,检出限为4.543×10-4g/（s·L）。

Study on Method for Determination of 1,15-Pentadecanedioic Acid by HPLC

A method of reversed phase high performance liquid chromatography （HPLC） with internal standard was applied to the quantitative analysis of 1,15-pentadecanedioic acid, which was an important material in the synthesis of cyclopentadecanolide. Optimum conditions were determined as follows： column packed with C18（4.6×250 mm）, lauric acid as internal standard.The mobile phase consists of a mixture of methanol, acetonitrile and water（80∶5∶15, 0.1%trifluoroacetic acid） with a flow rate of 1.2 mL/min and UV detection performed at 210nm. Desired relativity（r0.9990）and analytic precision （RSD 1% ） were obtained by the method with average recovery rate of 99.25% and sensitivity of 4.543×10-4 g/（s·L）.