| PEG/(NH4)2SO4双水相体系提取和纯化糖化酶 |
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| 引用本文： | 黄淑霞,吴晓莉,尹卓容. PEG/(NH4)2SO4双水相体系提取和纯化糖化酶[J]. 酿酒科技, 2003, 0(2): 24-25 |
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| 作者姓名： | 黄淑霞 吴晓莉 尹卓容 |
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| 作者单位： | 山东轻工业学院,山东,济南,250100 |
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| 摘 要： | 用PEG／（NH4）2SO4双水相体系提取，纯化糖化酶，研究了影响体系分配系数，回收率和纯化因子的因素从而确定了萃取糖化酶的最佳条件。在WPEG为0．24，W（NH4）2SO4为0．08条件下，分配系数最小，达0．022，回收率为95．6％。
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| 关 键 词： | 糖化酶 双水相体系 提取 纯化 |
| 文章编号： | 1001-9286(2003)02-0024-02 |
| 修稿时间： | 2002-11-11 |
Extraction and Purification of Saccharifying Enzymes by PEG/(NH4)2SO4 Double Aqueous Phases System |
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| HUANG Shu-xia,WU Xiao-li and YIN Zhuo-rong. Extraction and Purification of Saccharifying Enzymes by PEG/(NH4)2SO4 Double Aqueous Phases System[J]. Liquor-making Science & Technology, 2003, 0(2): 24-25 |
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| Authors: | HUANG Shu-xia WU Xiao-li YIN Zhuo-rong |
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| Abstract: | The saccharifying enzymes were extracted and purified by PEG/(NH 4 ) 2 SO 4 double aqueous phases system.The factors affect-ing system distribution coefficients,recycling rate and purifying agents were studied and the optimal conditions of saccharifying enzymes extraction were then confirmed.Under the conditions of W PEG as0.24and W (NH 4)2 SO 4 as0.08,the smallest distribution coefficient obtained as0.022and the recycling rate was95.6%.(Tran.by YUE Yang) |
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| Keywords: | saccharifying enzymes PEG/(NH 4 ) 2 SO 4 double aqueous phases system extraction purification |
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