Phenotypic and genetic evaluations of biogenic amine production by lactic acid bacteria isolated from fish and fish products |
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Authors: | Muñoz-Atienza Estefanía Landeta Gerardo de las Rivas Blanca Gómez-Sala Beatriz Muñoz Rosario Hernández Pablo E Cintas Luis M Herranz Carmen |
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Affiliation: | a Grupo de Seguridad y Calidad de los Alimentos por Bacterias Lácticas, Bacteriocinas y Probióticos (Grupo SEGABALBP), Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spainb Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de Alimentos y Nutrición, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain |
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Abstract: | In this work, biogenic amine production (histamine, tyramine and putrescine) by a collection of 74 lactic acid bacteria of aquatic origin has been investigated by means of amino acid decarboxylation by growth on decarboxylase differential medium, biogenic amine detection by thin-layer chromatography (TLC) and decarboxylase gene detection by PCR. None of the evaluated strains showed neither production of histamine and putrescine, nor presence of the genetic determinants encoding the corresponding decarboxylase activities. However, the tyrosine decarboxylase gene (tdc) was present in all the enterococcal strains, and tyramine production was detected by TLC in all of them but Enterococcus faecium BCS59 and MV5. Analysis of the tyrosine decarboxylase operon of these strains revealed the presence of an insertion sequence upstream tdc that could be responsible for their lack of tyrosine decarboxylase activity. |
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Keywords: | Fish Lactic acid bacteria Enterococci Fish probiotic Biogenic amine Tyramine |
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