Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat |
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Authors: | Hsouna Anis Ben Trigui Mohamed Mansour Riadh Ben Jarraya Raoudha Mezghani Damak Mohamed Jaoua Samir |
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Affiliation: | a Biopesticides Team (LPIP), Center of Biotechnology of Sfax, P.B. 1177, 3018 Sfax, Tunisiab Biotechnology and pathologies Unit, Higher Institute of Biotechnology of Sfax, B.P. 1175, 3038 Sfax, Tunisiac Laboratory of Natural Substances Chemistry, Faculty of Science, B.P.1171, 3000 Sfax, Tunisiad Qatar University, College of Arts & Sciences, P.O. Box. 2713. Doha, Qatar |
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Abstract: | The present study describes the phytochemical profile and the protective effects of Ceratonia siliqua pods essential oil (CsEO), a food and medicinal plant widely distributed in Tunisia. Twenty five different components were identified in the CsEO. Among them, the major detected components were: Nonadecane, Heneicosane , Naphthalene, 1,2-Benzenedicarboxylic acid dibutylester, Heptadecane, Hexadecanoic acid, Octadecanoic acid, 1,2-Benzenedicarboxylic acid, Phenyl ethyl tiglate, Eicosene, Farnesol 3, Camphor, Nerolidol and n-Eicosane. The antimicrobial activity of CsEO was evaluated against a panel of 13 bacteria and 8 fungal strains using agar diffusion and broth microdilution methods. Results have shown that CsEO exhibited moderate to strong antimicrobial activity against the tested species. In addition, the inhibitory effect of this CsEO was evaluated in vivo against a foodborne pathogens Listeria monocytogenes, experimentally inoculated in minced beef meat (2 × 102 CFU/g of meat) amended with different concentrations of the CsEO and stored at 7 °C for 10 days. The antibacterial activity of CsEO in minced beef meat was clearly evident and its presence led to a strong inhibitory effect against the pathogens at 7 °C. On the other hand, the cytotoxic effects of the essential oil against two tumoral human cell lines HeLa and MCF-7 were examined by MTT assay. The CsEO showed an inhibition of both cell lines with significantly stronger activity against HeLa cells. The IC50 values were 210 and 800 μg/ml for HeLa and MCF-7 cells, respectively. Overall, results presented here suggest that the EO of C. siliqua possesses antimicrobial and cytotoxic properties, and is therefore a potential source of active ingredients for food and pharmaceutical industry. |
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Keywords: | Ceratonia siliqua pods Essential oil Chemical composition Antimicrobial Cytotoxicity Meat preservation |
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