Proteomic analysis of responses of a new probiotic bacterium Lactobacillus casei Zhang to low acid stress |
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Authors: | Wu Rina Zhang Wenyi Sun Tiansong Wu Junrui Yue Xiqing Meng He Zhang Heping |
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Affiliation: | a College of Food Science, Shenyang Agricultural University, Shenyang 110866, PR Chinab The Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, PR Chinac School of Agricultural and biological, Shanghai Jiao Tong University, Shanghai 200240, PR China |
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Abstract: | Tolerance to acid is an important feature for probiotic bacteria during transition through the gastrointestinal tract. Proteomics analysis of a new probiotic bacterium, Lactobacillus casei Zhang, was performed upon 30-min exposure to low acid stress (pH 2.5 vs. pH 6.4) using two-dimensional electrophoresis. Out of 33 protein spots that showed changes of expression between the two pHs, 22 showed 1.5-fold higher expression at pH 2.5 than at pH 6.4, whereas five spots had expression decreased by 1.5-fold at pH 2.5. There were also six protein spots that were exclusively present on different pH maps. Further analysis showed that eight of the enhanced proteins, NagA, NagB, PGM, GlmM, LacC, TDP, GALM and PtsI, were involved in carbohydrate catabolism. Moreover, quantitative RT-PCR showed that the mRNA expression levels of dnaK, nagB, galm, estC, tuf and luxS were consistent with changes in protein expression. We postulate that there might be some relationship between differentially expressed proteins and acid tolerance in L. casei Zhang. |
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Keywords: | 2-DE, two dimensional electrophoresis SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis IPG, immobilized pH gradient MS, mass spectrometry MRS, de Man, Rogosa and Sharpe DTT, dithiothreitol COGs, clusters of orthologous groups ATR, acid tolerance response |
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