Characterization of the novel Listeria monocytogenes PCR serogrouping profile IVb-v1 |
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| Authors: | Leclercq Alexandre Chenal-Francisque Viviane Dieye Hélène Cantinelli Thomas Drali Rezak Brisse Sylvain Lecuit Marc |
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| Affiliation: | a Institut Pasteur, WHO Collaborating Centre and French National Reference Centre for Listeria, Microbes and Host Barriers Group, Paris, Franceb Service des Entérobactéries et Hygiène de l'Environnement, Laboratoire des Listeria, Institut Pasteur d'Algérie, Route du Petit Staouéli - Dély Brahim, Alger, Algeriac Institut Pasteur, Genotyping of Pathogens and Public Health, Paris, Franced Université Paris Descartes, Department of Infectious Diseases and Tropical Medicine, Necker-Pasteur Centre for Infectious Diseases, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France |
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| Abstract: | The World Health Organization Collaborating Centre for Listeria (WHOCCL) has developed in 2004 a multiplex PCR assay that separates the 4 major Listeria monocytogenes serovars (1/2a, 1/2b, 1/2c, and 4b) into distinct PCR serogroups. A new PCR profile has been recently identified, constituted of amplified DNA fragments of prs, ORF2819, ORF2110 and lmo0737. Here we characterize 22 L. monocytogenes isolates of the WHOCCL collection with this PCR IVb variant 1 (IVb-v1) profile. The 22 isolates belong to the clinically predominant serovar 4b, exhibit 6 distinct pulsed-field gel electrophoresis ApaI/AscI combined profiles, and belong to 2 unrelated multilocus sequence types, indicating that the novel profile does not correspond to a recent clonal emergence. We have updated the WHOCCL serogroup-related PCR typing scheme to include this new profile. |
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| Keywords: | PCR serogroup Listeria monocytogenes 4b MLST PFGE Atypical pattern |
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