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Graphene functionalized decellularized scaffold promotes skin cell proliferation
Authors:Mahboubeh Jafarkhani  Zeinab Salehi  Zahra Bagheri  Zahra Aayanifard  Ali Rezvan  Hamid Doosthosseini  Mohammad Ali Shokrgozar
Affiliation:1. School of Chemical Engineering, College of Engineering, University of Tehran, Iran

DTU Nanotech, Technical University of Denmark, Kongens, Lyngby, Region Hovedstaden, Denmark;2. School of Chemical Engineering, College of Engineering, University of Tehran, Iran;3. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

Abstract:An increasing number of new strategies for skin tissue engineering have been developed with the potential to mimic the biological properties of native tissue with a high degree of complexity, flexibility, and reproducibility. In this study, decellularized tissue (DT) was prepared from the bovine heart by using chemical treatments. However, the mechanical properties of the DT constructs were poorer than the extra cellular matrix of the skin tissue. To overcome this challenge, hybrid scaffolds of DT and graphene oxide (GO) were developed and the effects of the GO concentration on the morphology, pore size, porosity, mechanical strength, and water uptake capacity of the samples were evaluated. Moreover, the biocompatibility of hybrid scaffolds was studied by Live/Dead staining. The results show that a hybrid scaffold incorporating 3 % graphene oxide improved the mechanical strength and cell viability by ~25 % in comparison to the DT scaffolds. Cell viability results confirmed that the porous scaffolds could support cell adhesion, proliferation, and cell activity for 7 days. This study provides new insight into and opportunities for using graphene-based materials to develop biomimetic constructs for clinical applications.
Keywords:decellularized tissues  biomimetic scaffolds  graphene oxide  skin tissue engineering
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