| Affiliation: | 1. Molecular Biotechnology, Department of Biosciences, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main, Germany
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, QianHuHouCun 1, 210014 Nanjing, China;2. Molecular Biotechnology, Department of Biosciences, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main, Germany;3. Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002 Basel, Switzerland |
| Abstract: | A new cyclic lipopeptide, phototemtide A ( 1 ), was isolated from Escherichia coli expressing the biosynthetic gene cluster pttABC from Photorhabdus temperata Meg1. The structure of 1 was elucidated by HR-ESI-MS and NMR experiments. The absolute configurations of amino acids and 3-hydroxyoctanoic acid in 1 were determined by using the advanced Marfey's method and comparison after total synthesis of 1 , respectively. Additionally, three new minor derivatives, phototemtides B–D ( 2 – 4 ), were identified by detailed HPLC–MS analysis. Phototemtide A ( 1 ) showed weak antiprotozoal activity against Plasmodium falciparum, with an IC50 value of 9.8 μm . The biosynthesis of phototemtides A–D ( 1 – 4 ) was also proposed. |
