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Study of Protein Adsorption During Sterile Filtration of Protein Formulations by ILC |
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| Authors: | Susanne Maria Hilda Haindl Olivia Doppleb Lucas Förster Sophia Wraage Annette Reiche |
| Affiliation: | 1. Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37089 Göttingen, Germany;2. Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37089 Göttingen, Germany
Clausthal University of Technology, Institut für Physikalische Chemie, Arnold-Sommerfeld-Straße 4, 38678 Clausthal-Zellerfeld, Germany;3. Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37089 Göttingen, Germany Georg-August-Universität Göttingen, Institut für Organische und Biomolekulare Chemie, Tammannstraße 2, 37077 Göttingen, Germany |
| Abstract: | Protein adsorption is usually regarded as the main reason for filter fouling in sterile filtration of protein formulations. To achieve a better insight into this phenomenon, protein adsorption was studied during filtration of stabilized bovine serum albumin (BSA) and γ-globulin formulations through 0.2-µm microfilter membranes by inverse liquid chromatography (ILC). Adsorption processes can be studied with this method by measurement of breakthrough curves. The change of the concentration in the fluid phase is measured with high accuracy by an inline UV-detector. |
| Keywords: | Inverse liquid chromatography Protein adsorption Sterile filtration |