| 13C呼吸试剂D-木糖-U-13C5的合成 |
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| 引用本文： | 徐仲杰,卢伟京,卢浩. 13C呼吸试剂D-木糖-U-13C5的合成[J]. 同位素, 2012, 25(4): 209-213. DOI: 10.7538/tws.2012.25.04.0209 |
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| 作者姓名： | 徐仲杰 卢伟京 卢浩 |
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| 作者单位： | 上海化工研究院，上海200062 |
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| 基金项目： | 国家高新技术研究发展计划项目(2008AA02Z427)项目负责人:徐大刚,硕士,E-mail:shykjc@126.com教授 |
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| 摘 要： | 以D-葡萄糖-U-13C6为起始原料，采用减碳法经双丙酮保护、选择性酸水解、氧化、还原和碱水解五步合成D-木糖-U-13C5，总产率为38.6%（以D-葡萄糖-U-13C6计）。对每步产物使用相应的气相色谱（GC）、气质联用仪（GC-MS）、液相色谱（HPLC）和液质联用仪（LC-MS）进行表征。分析结果表明，每步反应中的产物均为目的产物，同位素丰度和纯度都符合要求，最后一步的产物为D-木糖-U-13C5，其13C丰度为99.1 % ，化学纯度为99.9%，符合13C呼吸试验的要求。
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| 关 键 词： | D-木糖-U-13C5 13C呼吸试剂 合成 |
Synthesis of D-Xylose-U-13C5 |
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| XU Zhong-jie,LU Wei-jing,LU Hao. Synthesis of D-Xylose-U-13C5[J]. Isotopes, 2012, 25(4): 209-213. DOI: 10.7538/tws.2012.25.04.0209 |
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| Authors: | XU Zhong-jie LU Wei-jing LU Hao |
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| Affiliation: | Shanghai Research Institute of Chemical Industry, Shanghai 200062, China |
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| Abstract: | D-Xylose-U-13C5 was synthesized with D-glucose-U-13C6 as starting material via process of protection with double acetone, selective hydrolysis with acid, oxidation, reduction and hydrolysis with base. The total yield of D-Xylose-U-13C5 was 38.6% base on D-glucose-U-13C6. The target compound and their intermediates were analyzed and characterized by GC, GC-MS, HPLC and LC-MS, accordingly. The results showed that their isotopic abundance and purity could meet the required specification. The structure of final product was D-Xylose-U-13C5, 13C abundance was 99.1%， and chemical purity was 99.9%, which were high enough for use in 13C breath test. |
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| Keywords: | D-Xylose-U-13C5 13C breath test synthesis |
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